| Reference : ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity. |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/10993/6277 | |||
| ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity. | |
| English | |
| Fradelizi, J. [> >] | |
| Noireaux, V. [> >] | |
| Plastino, J. [> >] | |
| Menichi, B. [> >] | |
| Louvard, D. [> >] | |
| Sykes, C. [> >] | |
| Golsteyn, R. M. [> >] | |
Friederich, Evelyne  [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| 2001 | |
| Nature Cell Biology | |
| 3 | |
| 8 | |
| 699-707 | |
| Yes (verified by ORBilu) | |
| International | |
| 1465-7392 | |
| England | |
| [en] Actin Cytoskeleton/drug effects/metabolism/ultrastructure ; Actin-Related Protein 2 ; Actin-Related Protein 3 ; Actins/metabolism ; Animals ; Bacterial Proteins/metabolism ; Biological Assay ; Cell Adhesion Molecules/metabolism ; Cell-Free System ; Cercopithecus aethiops ; Cytoskeletal Proteins ; Fluorescent Antibody Technique ; Glycoproteins ; HeLa Cells/cytology/drug effects/metabolism ; Humans ; Membrane Proteins/metabolism ; Metalloproteins/genetics/metabolism ; Microfilament Proteins ; Microspheres ; Mitochondria/metabolism/ultrastructure ; Phosphoproteins/metabolism ; Polymers/metabolism ; Proteins/metabolism ; Recombinant Proteins/metabolism ; Transfection ; Vero Cells/cytology/drug effects/metabolism ; Wiskott-Aldrich Syndrome Protein ; Zyxin | |
| [en] The actin cytoskeleton is a dynamic network that is composed of a variety of F-actin structures. To understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. We found that human zyxin and the related protein ActA of Listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (VASP) manner, but independently of the Arp2/3 complex. These results are consistent with the concept that there are multiple actin-polymerization machines in cells. With these simple tests it is possible to probe the specific function of proteins or identify novel molecules that act upon cellular actin polymerization. | |
| http://hdl.handle.net/10993/6277 | |
| 10.1038/35087009 |
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