| Reference : Effect of SK&F 96365 on extracellular Ca2+ -dependent O2- production in neutrophil-li... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/10993/5726 | |||
| Effect of SK&F 96365 on extracellular Ca2+ -dependent O2- production in neutrophil-like HL-60 cells | |
| English | |
| Gallois, A. [> >] | |
Bueb, Jean-Luc  [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| Tschirhart, Eric [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| 1999 | |
| European Journal of Pharmacology | |
| Elsevier Science | |
| 361 | |
| 2-3 | |
| 293-8 | |
| Yes (verified by ORBilu) | |
| 0014-2999 | |
| Amsterdam | |
| The Netherlands | |
| [en] drug effects ; Calcium Channel Blockers ; HL-60 Cells ; Humans ; Imidazoles ; Manganese ; N-Formylmethionine Leucyl-Phenylalanine ; Neutrophils ; Oxygen ; Respiratory Burst ; pharmacology ; metabolism ; Calcium | |
| [en] Store-operated Ca2+ entry is referred to a capacitative current activated by Ca2+ -stores depletion in various non-excitable cells. Neutrophil-like HL-60 cells responded to N-formyl-L-Methionyl-L-Leucyl-L-Phenylalanine (fMLP) by an early O2- production preceded by a [Ca2+]i rise. Cell stimulation in the absence of extracellular Ca2+ resulted in a major reduction of [Ca2+]i rise and O2- production. A purported inhibitor of store-operated Ca2+ entry, SK&F 96365 (1-(beta-(3-(4-methoxy-phenyl)propoxyl)-4-methoxy-phenetyl)- 1H-imidazole hydrochloride), inhibited extracellular Ca2+ -dependent [Ca2+]i rise by 30% but did not alter O2- production. In conclusion, SK&F 96365 did not modify extracellular Ca2+ -dependent O2- production, despite a significant but limited reduction in fMLP-activated membrane Ca2+ fluxes which can be ascribed to store-operated Ca2+ entry. Furthermore, Ca2+ influx is necessary for a full induction and maintenance of the biological response. | |
| http://hdl.handle.net/10993/5726 |
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