| Reference : Rac-1-mediated O2- secretion requires Ca2+ influx in neutrophil-like HL-60 cells |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/10993/5723 | |||
| Rac-1-mediated O2- secretion requires Ca2+ influx in neutrophil-like HL-60 cells | |
| English | |
| Valentin, F. [> >] | |
Bueb, Jean-Luc  [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| Capdeville-Atkinson, C. [> >] | |
| Tschirhart, Eric [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| 2001 | |
| Cell Calcium | |
| Churchill Livingstone, Inc. | |
| 29 | |
| 6 | |
| 409-15 | |
| Yes (verified by ORBilu) | |
| 0143-4160 | |
| Edinburgh | |
| Scotland | |
| [en] Calcium ; drug effects ; pharmacology ; metabolism ; agonists ; rac1 GTP-Binding Protein ; Virulence Factors, Bordetella ; Signal Transduction ; Reactive Oxygen Species ; Pertussis Toxin ; NADPH Oxidase ; N-Formylmethionine Leucyl-Phenylalanine ; Inositol Phosphates ; Humans ; HL-60 Cells ; GTP-Binding Proteins ; Extracellular Space ; Cell Membrane ; physiology | |
| [en] Neutrophil-like HL-60 cells reacted to N -formyl- l -Methionyl- l -Leucyl- l -P henylalanine (f MLP) with a rise in the intracellular calcium concentration ([Ca2]i), NADPH oxidase activation, and increased superoxide anion (O2-) production. [Ca2+]i mobilization and superoxide production were largely dependent on extracellular calcium (Ca2+]e) and a capacitative calcium entry. The monomeric G-protein, Rac-1, regulates NADPH oxidase activity. We tested the effect of removal of Ca2+]e on Rac-1 plasma membrane sequestration and activation of NADPH oxidase using immunodetection and a double labelling fluorescent method. Results showed that Rac-1 activation is mediated via a pertussis toxin (PTX)-sensitive heteromeric G-protein pathway, and that Rac-1 membrane sequestration was preceded by [Ca2+]i mobilization following entry of Ca2+ e. Therefore, we propose that O2- production is dependent on activation of PTX-sensitive G-proteins and sequestration of Rac-1 in the plasma membrane, following entry of Ca2+ e. | |
| http://hdl.handle.net/10993/5723 | |
| 10.1054/ceca.2001.0203 |
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