| Reference : Interleukin-8 primes oxidative burst in neutrophil-like HL-60 through changes in cyto... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/10993/5719 | |||
| Interleukin-8 primes oxidative burst in neutrophil-like HL-60 through changes in cytosolic calcium | |
| English | |
Bréchard, Sabrina  [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| Bueb, Jean-Luc [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| Tschirhart, Eric [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >] | |
| 2005 | |
| Cell Calcium | |
| Churchill Livingstone, Inc. | |
| 37 | |
| 6 | |
| 531-40 | |
| Yes (verified by ORBilu) | |
| 0143-4160 | |
| Edinburgh | |
| Scotland | |
| [en] drug effects ; Fura-2 ; HL-60 Cells ; Humans ; Hydrogen Peroxide ; Interleukin-8 ; N-Formylmethionine Leucyl-Phenylalanine ; Neutrophils ; Oxazines ; Reactive Oxygen Species ; Respiratory Burst ; physiology ; metabolism ; pharmacology ; Calcium | |
| [en] In response to a variety of stimuli, neutrophils release large amount of reactive oxygen species (ROS) generated by NADPH oxidase. This process known as the respiratory burst is dependent on cytosolic free calcium concentration ([Ca(2+)](i)). Proinflammatory cytokines such as interleukin-8 (IL-8) may modulate ROS generation through a priming phenomenon. The aim of this study was to determine the effect of human IL-8 on ROS production in neutrophil-like dimethylsulfoxide-differentiated HL-60 cells (not equalHL-60 cells) and further to examine the role of Ca(2+) mobilization during the priming. IL-8 at 10 nM induced no ROS production but a [Ca(2+)](i) rise (254 +/- 36 nM). IL-8 induced a strongly enhanced (2 fold) ROS release during stimulation with 1 microM of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLF). This potentiation of ROS production is dependent of extracellular Ca(2+) (17.0+/-4.5 arbitrary units (A.U.) in the absence of Ca(2+) versus 56.6 +/- 3.9 A.U. in the presence of 1.25 mM of Ca(2+)). Also, IL-8 enhanced fMLF-stimulated increase in [Ca(2+)](i) (375 +/- 35 versus 245 +/- 21 nM, 0.1 microM of fMLF). IL-8 had no effect on not equalHL-60 cells in response to 1 microM of thapsigargin (472 +/- 66 versus 470 +/- 60 nM). In conclusion, Ca(2+) influx is necessary for a full induction of neutrophil priming by IL-8. | |
| http://hdl.handle.net/10993/5719 | |
| 10.1016/j.ceca.2005.01.019 |
There is no file associated with this reference.
All documents in ORBilu are protected by a user license.
