Reference : Generation of two induced pluripotent stem cell lines and the corresponding isogenic ...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/10993/55642

Title :	Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G>A (p.R272Q) or c.1348C>T (p.R450C) in the RHOT1 gene encoding Miro1
Language :	English
Author, co-author :	Chemla, Axel [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
	Arena, Giuseppe [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
	Onal, Gizem [> >]
	Walter, Jonas [> >]
	Berenguer-Escuder, Clara [> >]
	Grossmann, Dajana [> >]
	Grünewald, Anne [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Molecular and Functional Neurobiology]
	Schwamborn, Jens Christian [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Developmental and Cellular Biology]
	Krüger, Rejko [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
Publication date :	2023
Journal title :	Stem Cell Research
Pages :	103145
Peer reviewed :	Yes
ISSN :	1873-5061
Abstract :	[en] Fibroblasts from two Parkinson’s disease (PD) patients carrying either the heterozygous mutation c.815G>A (Miro1 p.R272Q) or c.1348C>T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).
Target :	Researchers
Permalink :	http://hdl.handle.net/10993/55642
DOI :	10.1016/j.scr.2023.103145
Other URL :	https://www.sciencedirect.com/science/article/pii/S1873506123001319
FnR project :	FnR ; FNR11676395 > Rejko Krüger > MiRisk-PD > Mitochondrial Risk Factors In Parkinson's Disease > 01/03/2018 > 31/08/2021 > 2017

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