Reference : Accurate long-read sequencing identified GBA variants as a major genetic risk factor ...

	Document type :	E-prints/Working papers : Already available on another site
	Discipline(s) :	Life sciences : Genetics & genetic processes Human health sciences : Neurology
	Focus Areas :	Systems Biomedicine
	To cite this reference:	http://hdl.handle.net/10993/55244

Title :	Accurate long-read sequencing identified GBA variants as a major genetic risk factor in the Luxembourg Parkinson\textquoterights study 2023.03.29.23287880
Language :	English
Author, co-author :	Peiris, Sinthuja [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
	Landoulsi, Zied [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Bioinformatics Core]
	Pavelka, Lukas [University of Luxembourg > >]
	Schulte, Claudia [> >]
	Buena-Atienza, Elena [> >]
	Gross, Caspar [> >]
	Hauser, Ann-Kathrin [> >]
	Bobbili, Dheeraj Reddy [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Bioinformatics Core]
	Casadei, Nicolas [> >]
	May, Patrick [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Bioinformatics Core]
	Krüger, Rejko [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > Translational Neuroscience]
	Consortium, The NCER-PD [> >]
Publication date :	29-Mar-2023
Publisher :	Cold Spring Harbor Laboratory Press
Collection and collection volume :	medRxiv 2023.03.29.23287880
Peer reviewed :	No
Keywords :	[en] GBA ; Parkinson's disease ; Long-read sequencing ; Case-control cohort
Abstract :	[en] Heterozygous variants in the glucocerebrosidase GBA gene are an increasingly recognized risk factor for Parkinson's disease (PD). Due to the pseudogene GBAP1 that shares 96\% sequence homology with the GBA coding region, accurate variant calling by array-based or short-read sequencing methods remains a major challenge in understanding the genetic landscape of GBA-related PD. We established a novel long-read sequencing technology for assessing the full length of the GBA gene. We used subsequent regression models for genotype-phenotype analyses. We sequenced 752 patients with parkinsonism and 806 healthy controls of the Luxembourg Parkinson's study. All GBA variants identified showed a 100% true positive rate by Sanger validation. We found 12% of unrelated PD patients carrying GBA variants. Three novel variants of unknown significance (VUS) were identified. Using a structure-based approach, we defined a potential risk prediction method for VUS. This study describes the full landscape of GBA-related parkinsonism in Luxembourg, showing a high prevalence of GBA variants as the major genetic risk for PD. Our approach provides an important advancement for highly accurate GBA variant calling, which is essential for providing access to emerging causative therapies for GBA carriers.
Research centres :	Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group) ; Luxembourg Centre for Systems Biomedicine (LCSB): Clinical & Experimental Neuroscience (Krüger Group) ; Luxembourg Institute of Health - LIH
Funders :	Fonds National de la Recherche - FnR
Name of the research project :	NCER-PD
Target :	Researchers ; Professionals
Permalink :	http://hdl.handle.net/10993/55244
DOI :	10.1101/2023.03.29.23287880
Other URL :	https://www.medrxiv.org/content/early/2023/03/30/2023.03.29.23287880
source URL :	https://www.medrxiv.org/
FnR project :	FnR ; FNR11264123 > Rejko Krüger > NCER-PD > Ncer-pd > 01/01/2015 > 30/11/2020 > 2015

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	Fulltext file(s): File Commentary Version Size Access Open access 2023.03.29.23287880v2.full.pdf Publisher postprint 1.55 MB View/Open

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