Reference : Molecular cloning and expression analysis of Pleurodeles waltl complement component C...

	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/10993/27282

Title :	Molecular cloning and expression analysis of Pleurodeles waltl complement component C3 under normal physiological conditions and environmental stresses
Language :	English
Author, co-author :	Gueguinou, Nathan [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit]
	Huin-Schohn, Cécile [Lorraine University, Faculty of Medicine, Stress Immunity Pathogens Laboratory, EA7300, 9 Avenue de la Forêt de Haye, F-54500 Vandœuvre-lès-Nancy, France]
	Ouzren-Zarhloul, Nassima [Lorraine University, Faculty of Medicine, Stress Immunity Pathogens Laboratory, EA7300, 9 Avenue de la Forêt de Haye, F-54500 Vandœuvre-lès-Nancy, France]
	Ghislin, Stéphanie [Lorraine University, Faculty of Medicine, Stress Immunity Pathogens Laboratory, EA7300, 9 Avenue de la Forêt de Haye, F-54500 Vandœuvre-lès-Nancy, France]
	Frippiat, Jean-Pol [Lorraine University, Faculty of Medicine, Stress Immunity Pathogens Laboratory, EA7300, 9 Avenue de la Forêt de Haye, F-54500 Vandœuvre-lès-Nancy, France]
Publication date :	2014
Journal title :	Developmental and Comparative Immunology
Publisher :	Elsevier Ltd
Volume :	46
Issue/season :	2
Pages :	180-185
Peer reviewed :	Yes
Audience :	International
ISSN :	0145305X
Keywords :	[en] Complement component ; Environment ; Pleurodeles waltl ; Stress ; Urodele amphibian ; Pleurodeles ; Pleurodeles ; Amino Acid Sequence ; Amphibian Proteins ; Animals ; Cloning, Molecular ; Complement C3 ; Gene Expression ; Gene Expression Regulation ; Hydrogen-Ion Concentration ; Molecular Sequence Data ; Organ Specificity ; Phylogeny ; Pleurodeles ; Stress, Physiological
Abstract :	[en] C3 is a component of the complement system that plays a central role in immunity, development and tissue regeneration. In this study, we isolated the C3 cDNA of the Iberian ribbed newt Pleurodeles waltl. This cDNA encodes a 1637 amino acid protein with an estimated molecular mass of 212.5. kDa. The deduced amino acid sequence showed that P. waltl C3 contains all the conserved domains known to be critical for C3 function. Quantitative real-time PCR (qRT-PCR) demonstrated that under normal physiological conditions, P. waltl C3 mRNA is expressed early during development because it is likely required for neurulation. Then, its expression increased as the immune system developed. In adults. the liver is the richest source of C3, though other tissues can also contribute. Further analysis of C3 expression demonstrated that C3 transcription increased when P. waltl larvae were exposed to pH or temperature stress, suggesting that environmental modifications might affect this animal's defenses against pathogens. © 2014 Elsevier Ltd.
Permalink :	http://hdl.handle.net/10993/27282
DOI :	10.1016/j.dci.2014.04.011

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