Reference : A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biotechnology Life sciences : Microbiology Life sciences : Multidisciplinary, general & others Human health sciences : Laboratory medicine & medical technology
	Focus Areas :	Systems Biomedicine
	To cite this reference:	http://hdl.handle.net/10993/27053

Title :	A microfluidics-based in vitro model of the gastrointestinal human-microbe interface.
Language :	English
Author, co-author :	Shah, Pranjul [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
	Fritz, Joëlle [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Glaab, Enrico [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
	Desai, Mahesh S. [> >]
	Greenhalgh, Kacy [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
	Frachet Bour, Audrey [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
	Niegowska, Magdalena [> >]
	Estes, Matthew [> >]
	Jäger, Christian [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
	Seguin-Devaux, Carole [> >]
	Zenhausern, Frederic [> >]
	Wilmes, Paul [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) >]
Publication date :	2016
Journal title :	Nature communications
Publisher :	Nature Pub.lishing Group
Volume :	7
Pages :	11535
Peer reviewed :	Yes (verified by ORBilu)
Audience :	International
ISSN :	2041-1723
e-ISSN :	2041-1723
City :	London
Country :	England
Keywords :	[en] microfluidics ; microbiome ; co-culture ; gastrointestinal ; model ; microarray analysis ; confocal microscopy ; PCR
Abstract :	[en] Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host-microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.
Research centres :	Luxembourg Centre for Systems Biomedicine (LCSB): Eco-Systems Biology (Wilmes Group)
Funders :	Fonds National de la Recherche Luxembourg - FNR
Name of the research project :	R-STR-4014-00 > Eco-Systems Biology (Wilmes) > 01/01/2011 - 19/01/2048 > WILMES Paul
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/10993/27053
	also: http://hdl.handle.net/10993/27382
DOI :	10.1038/ncomms11535
Other URL :	http://www.nature.com/ncomms/2016/160511/ncomms11535/full/ncomms11535.html
Mentions required by the publisher for OA :	The original publication is available at http://www.nature.com/ncomms/2016/160511/ncomms11535/full/ncomms11535.html
FnR project :	FNR ; FNR11014639 > Paul Wilmes > HuMiX2.0 > A microfluidics-based drug discovery platform emulating the human microbiome on chip > 01/09/2015 > 31/12/2016 > 2015

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Open access humix2016postprint.pdf Author postprint 13.92 MB View/Open

All documents in ORBi^lu are protected by a user license.

 

University of Luxembourg Library |  Feedback |  Legal notices Site Map