Reference : Dynamic regulation of microRNA expression following interferonγ- induced gene transcr...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/10993/20247

Title :	Dynamic regulation of microRNA expression following interferonγ- induced gene transcription
Language :	English
Author, co-author :	Reinsbach, Susanne [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Nazarov, Petr V. [Microarray Center, Centre de Recherche Public de la Santé, Luxembourg, Luxembourg]
	Philippidou, Demetra [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Schmitt, Martina [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit > > Signal Transduction Laboratory]
	Wienecke, Anke [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Muller, Arnaud [Centre de Recherche Public de la Santé Luxembourg > Microarray Center]
	Vallar, Laurent [Centre de Recherche Public de la Santé Luxembourg > Microarray Center]
	Behrmann, Iris [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
	Kreis, Stephanie [University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit >]
Publication date :	2012
Journal title :	RNA Biology
Volume :	9
Issue/season :	7
Pages :	987-989
Peer reviewed :	Yes
Audience :	International
ISSN :	1547-6286
e-ISSN :	1555-8584
Keywords :	[en] Dynamic expression; MicroRNA; Time-series microarrays; Transcriptional regulation
Abstract :	[en] MicroRNAs are major players in post-transcriptional gene regulation. Even small changes in miRNA levels may have profound consequences for the expression levels of target genes. Hence, miRNAs themselves need to be tightly, albeit dynamically, regulated. Here, we investigated the dynamic behavior of miRNAs over a wide time range following stimulation of melanoma cells with interferonγ (IFNγ), which activates the transcription factor STAT1. By applying several bioinformatic and statistical software tools for visualization and identification of differentially expressed miRNAs derived from time-series microarray experiments, 8.9% of 1105 miRNAs appeared to be directly or indirectly regulated by STAT1. Focusing on distinct dynamic expression patterns, we found that the majority of robust miRNA expression changes occurred in the intermediate time range (24-48 h). Three miRNAs (miR-27a, miR-30a and miR-34a) had a delayed regulation occurring at 72 h while none showed significant expression changes at early time points between 30 min and 6 h. Expression patterns of individual miRNAs were altered gradually over time or abruptly increased or decreased between two time points. Furthermore, we observed coordinated dynamic transcription of most miRNA clusters while few were found to be regulated independently of their genetic cluster. Most interestingly, several "star" or passenger strand sequences were specifically regulated over time while their "guide" strands were not. © 2012 Landes Bioscience.
Funders :	University of Luxembourg (F1R-LSC-PUL-09MIRN) ; Fondation Cancer (Luxembourg)
Permalink :	http://hdl.handle.net/10993/20247
DOI :	10.4161/rna.20494
Other URL :	http://www.scopus.com/inward/record.url?eid=2-s2.0-84865278613&partnerID=40&md5=b7eb9c38273f11f78547bb47194ecac6
Commentary :	cited By (since 1996)6 Scopus

	File(s) associated to this reference
	Fulltext file(s): File Commentary Version Size Access Limited access Reinsbach et al., 2012.pdf Publisher postprint 1.87 MB Request a copy

All documents in ORBi^lu are protected by a user license.

 

University of Luxembourg Library |  Feedback |  Legal notices Site Map