| Reference : Asparagine and glutamine metabolism in chicks. |
| Scientific journals : Article | |||
| Life sciences : Genetics & genetic processes | |||
| http://hdl.handle.net/10993/1249 | |||
| Asparagine and glutamine metabolism in chicks. | |
| English | |
| Coon, C. [> >] | |
Balling, Rudi  [] | |
| 1984 | |
| Poultry Science | |
| 63 | |
| 4 | |
| 717-29 | |
| Yes (verified by ORBilu) | |
| 0032-5791 | |
| UNITED STATES | |
| [en] Amino Acids/administration & dosage ; Ammonium Chloride/administration & dosage ; Animals ; Asparaginase/metabolism ; Asparagine/administration & dosage/metabolism ; Bicarbonates/administration & dosage ; Chickens/metabolism ; Dietary Proteins/administration & dosage ; Glutaminase/metabolism ; Glutamine/administration & dosage/metabolism ; Kidney/enzymology ; Liver/enzymology ; Mitochondria/enzymology ; Mitochondria, Liver/enzymology ; Sodium Bicarbonate | |
| [en] In a series of four experiments, asparaginase and glutaminase activity was measured in liver and kidney tissue of 7- to 19-day-old male broiler chicks. In Experiment 1, chicks were fed purified amino acid diets with 14.8 and 44.6% protein equivalents (PE) with 1, 3, or 5% added sodium bicarbonate. In Experiments 2, 3, and 4 the chicks were fed a 23% protein basal control diet, basal diet containing 5% ammonium chloride, and basal diet containing 5% ammonium chloride with 5 or 10% sodium bicarbonate, asparagine, or glutamine. In Experiments 2 and 4 the chicks were also fed 25, 50, or 75% protein-isolated soy-purified diets. The 44.6% PE diet increased liver and kidney asparaginase activity in chicks as compared to chicks fed a 14.8% PE diet. The addition of sodium bicarbonate to the 44.6% PE amino acid diet decreased the kidney asparaginase activity equivalent to kidney asparaginase activity of chicks fed the 14.8% PE diet. Asparaginase activity increased 4-fold in the kidneys of chicks fed the 23% protein basal diet containing 5% ammonium chloride and the pH of the urine from the chicks was 4.9. Chicks fed basal diets with 5% ammonium chloride plus 10% sodium bicarbonate or asparagine had the same kidney asparaginase activity and urine pH as chicks fed the 23% protein basal control diet. Glutamine added to chick diets containing 5% ammonium chloride did not decrease the kidney asparaginase activity or the urine acidity. Liver asparaginase activity was not increased in acidotic chicks fed diets with 5% ammonium chloride. The asparaginase activity of liver and kidney tissue were both significantly increased in chicks fed 75% protein-isolated soy purified diets and the pH of their urine was 5.6. The increase in liver asparaginase of chicks fed 75% protein or 44.5% PE diets was probably due to an endocrine gluconeogenic response producing increased catabolism of the majority of amino acids. The increase in kidney asparaginase of chicks fed 75% protein, 44.5% PE diets, and 23% protein basal diets with 5% ammonium chloride was primarily related to metabolic acidosis. Phosphate-dependent glutaminase (PDG) activity was localized in chick kidney mitochondria and was heat sensitive (55 C for 30 sec). The phosphate-independent glutaminase (PIG) activity was primarily localized in chick kidney mitochondria but was stable to a temperature of 55 C for 30 sec.(ABSTRACT TRUNCATED AT 400 WORDS) | |
| http://hdl.handle.net/10993/1249 |
There is no file associated with this reference.
All documents in ORBilu are protected by a user license.
