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Showing results 3441 to 3448 of 3448
   Review: Frank Steinbeck (2012). Das Motorrad. Ein deutscher Sonderweg in die automobile Gesellschaft. Stuttgart: Franz Steiner VerlagKrebs, Stefan  in Technology & Culture (2014), 55(1), 273-274 Detailed reference viewed: 135 (2 UL)Workshop: Ethik des Lebens und des Begehrens im LehrerberufWeber, Jean-Marie Conference given outside the academic context (2014) Detailed reference viewed: 48 (0 UL)Community integrated omics links the dominance of a microbial generalist to fine-tuned resource usageMuller, Emilie ; ; Laczny, Cedric Christian et alPoster (2014) Microbial communities are complex and dynamic systems that are influenced by stochastic-neutral processes but are mainly structured by resource availability and usage. High-resolution “meta-omics” offer ... [more ▼] Microbial communities are complex and dynamic systems that are influenced by stochastic-neutral processes but are mainly structured by resource availability and usage. High-resolution “meta-omics” offer exciting prospects to investigate microbial populations in their native environment. In particular, integrated meta-omics, by allowing simultaneous resolution of fundamental niches (genomics) and realised niches (transcriptomics, proteomics and metabolomics), can resolve microbial lifestyles strategies (generalist versus specialist) in situ. We have recently developed the necessary wet- and dry-lab methodologies to carry out systematic molecular measurements of microbial consortia over space and time, and to integrate and analyse the resulting data at the population-level. We applied these methods to oleaginous mixed microbial communities located on the surface of anoxic biological wastewater treatment tanks to investigate how niche breadth (generalist versus specialist strategies) relates to community-level phenotypes and ecological success (i.e. population size). Coupled metabolomics and 16S rRNA gene-based deep sequencing demonstrate that the community-wide lipid accumulation phenotype is associated with the dominance of Candidatus Microthrix parvicella. By integrating population-level genomic reconstructions with transcriptomic and proteomic data, we found that the dominance of this microbial generalist population results from finely tuned resource usage and optimal foraging behaviour. Moreover, the fluctuating environmental conditions constrain the accumulation of variations, leading to a genetically homogeneous population likely due to fitness trade-offs. By integrating metagenomic, metatranscriptomic, metaproteomic and metabolomic information, we demonstrate that natural microbial population sizes and structures are intricately linked to resource usage and that differing microbial lifestyle strategies may explain the varying degrees of within-population genetic heterogeneity observed in metagenomic datasets. Elucidating the exact mechanism driving fitness trade-offs, e.g., antagonistic pleiotropy or others, will require additional integrated omic datasets to be generated from samples taken over space and time. Based on our observations, niche breadth and lifestyle strategies (generalists versus specialists) have to be considered as important factors for understanding the evolutionary processes governing microbial population sizes and structures in situ. [less ▲] Detailed reference viewed: 171 (12 UL) Akustisches KapitalHesse, Markus in Zeitschrift für Wirtschaftsgeographie (2014), 58(1), 249-250 Detailed reference viewed: 78 (0 UL)L’application du nouveau droit des sociétés coopératives en période transitoireHiez, David in Revue du Droit des Affaires en Afrique (2014) Detailed reference viewed: 93 (11 UL)Acute depletion of endothelial beta3-integrin transiently inhibits tumor growth and angiogenesis in mice.; ; et al in Circulation Research (2014), 114(1), 79-91 RATIONALE: The dramatic upregulation of alphavbeta3-integrin that occurs in the vasculature during tumor growth has long suggested that the endothelial expression of this molecule is an ideal target for ... [more ▼] RATIONALE: The dramatic upregulation of alphavbeta3-integrin that occurs in the vasculature during tumor growth has long suggested that the endothelial expression of this molecule is an ideal target for antiangiogenic therapy to treat cancer. This discovery led to the development of small-molecule inhibitors directed against alphavbeta3-integrin that are currently in clinical trials. In 2002, we reported that beta3-integrin-knockout mice exhibit enhanced tumor growth and angiogenesis. However, as beta3-integrin is expressed by a wide variety of cells, endothelial cell-specific contributions to tumor angiogenesis are muddied by the use of a global knockout of beta3-integrin function. OBJECTIVE: Our aim was to examine the endothelial-specific contribution beta3-integrin makes to tumor growth and angiogenesis. METHODS AND RESULTS: We have crossed beta3-integrin-floxed (beta3-floxed) mice to 2 endothelial-specific Cre models and examined angiogenic responses in vivo, ex vivo, and in vitro. We show that acute depletion of endothelial beta3-integrin inhibits tumor growth and angiogenesis preventatively, but not in already established tumors. However, the effects are transient, and long-term depletion of the molecule is ineffective. Furthermore, long-term depletion of the molecule correlates with many molecular changes, such as reduced levels of focal adhesion kinase expression and a misbalance in focal adhesion kinase phosphorylation, which may lead to a release from the inhibitory effects of decreased endothelial beta3-integrin expression. CONCLUSIONS: Our findings imply that timing and length of inhibition are critical factors that need to be considered when targeting the endothelial expression of beta3-integrin to inhibit tumor growth and angiogenesis. [less ▲] Detailed reference viewed: 75 (1 UL)Mères monstrueuses dans les romans d’Anne Hébert. Réflexions sur les (dé)monstrations du genre fémininBarthelmebs-Raguin, Hélène in Amerika (2014) Detailed reference viewed: 101 (0 UL)Femmes et Suisses de langue française : territoire(s) en constructionBarthelmebs-Raguin, Hélène in Baudorre, Philippe; Casseville, Caroline (Eds.) Nouveaux Cahiers François Mauriac (2014) Detailed reference viewed: 31 (0 UL) |
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