Reference : Comparative Genomic Analysis of the Human Gut Microbiome Reveals a Broad Distribution...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Microbiology
Computational Sciences
http://hdl.handle.net/10993/32199
Comparative Genomic Analysis of the Human Gut Microbiome Reveals a Broad Distribution of Metabolic Pathways for the Degradation of Host-Synthetized Mucin Glycans and Utilization of Mucin-Derived Monosaccharides
English
Ravcheev, Dmitry mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
Thiele, Ines mailto [University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB) > >]
29-Aug-2017
Frontiers in Genetics
Frontiers
8
111
Yes (verified by ORBilu)
International
1664-8021
Lausanne
Switzerland
[en] human gut microbiome ; comparative genomics ; mucin glycans ; carbohydrate utilization ; metabolism reconstruction
[en] The colonic mucus layer is a dynamic and complex structure formed by secreted and transmembrane mucins, which are high-molecular-weight and heavily glycosylated proteins. Colonic mucus consists of a loose outer layer and a dense epithelium-attached layer. The outer layer is inhabited by various representatives of the human gut microbiota (HGM). Glycans of the colonic mucus can be used by the HGM as a source of carbon and energy when dietary fibers are not sufficiently available. Both commensals and pathogens can utilize mucin glycans. Commensals are mostly involved in the cleavage of glycans, while pathogens mostly utilize monosaccharides released by commensals. This HGM-derived degradation of the mucus layer increases pathogen susceptibility and causes many other health disorders. Here, we analyzed 397 individual HGM genomes to identify pathways for the cleavage of host-synthetized mucin glycans to monosaccharides as well as for the catabolism of the derived monosaccharides. Our key results are as follows: (i) Genes for the cleavage of mucin glycans were found in 86% of the analyzed genomes, which significantly higher than a previous estimation. (ii) Genes for the catabolism of derived monosaccharides were found in 89% of the analyzed genomes. (iii) Comparative genomic analysis identified four alternative forms of the monosaccharide-catabolizing enzymes and four alternative forms of monosaccharide transporters. (iv) Eighty-five percent of the analyzed genomes may be involved in potential feeding pathways for the monosaccharides derived from cleaved mucin glycans. (v) The analyzed genomes demonstrated different abilities to degrade known mucin glycans. Generally, the ability to degrade at least one type of mucin glycan was predicted for 81% of the analyzed genomes. (vi) Eighty-two percent of the analyzed genomes can form mutualistic pairs that are able to degrade mucin glycans and are not degradable by any of the paired organisms alone. Taken together, these findings provide further insight into the inter-microbial communications of the HGM as well as into host-HGM interactions.
National Research Fund Luxembourg
http://hdl.handle.net/10993/32199
10.3389/fgene.2017.00111
http://journal.frontiersin.org/article/10.3389/fgene.2017.00111
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The reviewer WY and handling Editor declared their shared affiliation.

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Open access
Ravcheev_Thiele_MucinGlycansHGM.pdfPublisher postprint4.16 MBView/Open

Additional material(s):

File Commentary Size Access
Open access
data sheet 1.fasta3.91 MBView/Open
Open access
image 1.pdf417.06 kBView/Open
Open access
image 2.pdf417.91 kBView/Open
Open access
image 3.pdf394.63 kBView/Open
Open access
image 4.pdf399.64 kBView/Open
Open access
image 5.pdf435.83 kBView/Open
Open access
image 6.pdf405.89 kBView/Open
Open access
image 7.pdf409.47 kBView/Open
Open access
image 8.pdf402.2 kBView/Open
Open access
image 10.pdf391.97 kBView/Open
Open access
image 11.pdf416.56 kBView/Open
Open access
table 1.xlsx48.74 kBView/Open
Open access
table 2.xlsx22.36 kBView/Open
Open access
table 3.xlsx62.82 kBView/Open
Open access
table 4.xlsx64.39 kBView/Open
Open access
table 5.xlsx71.35 kBView/Open
Open access
table 6.xlsx64.4 kBView/Open
Open access
table 7.xlsx96.2 kBView/Open
Open access
table 8.xlsx25.62 kBView/Open
Open access
table 9.xlsx21.92 kBView/Open
Open access
table 10.xlsx60.73 kBView/Open
Open access
table 11.xlsx376.85 kBView/Open
Open access
table 12.xlsx25.86 kBView/Open
Open access
table 13.xlsx56.6 kBView/Open
Open access
table 14.xlsx36.23 kBView/Open
Open access
table 15.xlsx106.4 kBView/Open

Bookmark and Share SFX Query

All documents in ORBilu are protected by a user license.