Article (Scientific journals)
The miRNome of Alzheimer's disease: consistent downregulation of the miR-132/212 cluster
Pichler, Sabrina; Gu, Wei; Hartl, Daniela et al.
2016In Neurobiology of Aging, 50, p. 167
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Keywords :
Alzheimer's disease; Human cortical brain tissue; microRNA
Abstract :
[en] MicroRNAs (miRNAs) are small noncoding RNA molecules, with essential functions in RNA silencing and post-transcriptional regulation of gene expression. miRNAs appear to regulate the development and function of the nervous system. Alterations of miRNA expression have been associated with Alzheimer's disease (AD). To characterize the AD miRNA signature, we examined genome-wide miRNA and mRNA expression patterns in the temporal cortex of AD and control samples. We validated our miRNA results by semiquantitative real-time polymerase chain reaction (PCR) in independent prefrontal cortex. Furthermore, we separated gray and white matter brain sections to identify the cellular origin of the altered miRNA expression. We observed genome-wide downregulation of hsa-miR-132-3p and hsa-miR-212-3p in AD with a stronger decrease in gray matter AD samples. We further identified 10 differently expressed transcripts achieving genome-wide levels of significance. Significantly deregulated miRNAs and mRNAs were correlated and examined for potential binding sites (in silico). This miRNome-wide study in AD provides supportive evidence and corroborates an important contribution of miR-132/212 and corresponding target mRNAs to the pathogenesis of AD.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Bioinformatics Core (R. Schneider Group)
Disciplines :
Neurology
Author, co-author :
Pichler, Sabrina
Gu, Wei ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Hartl, Daniela
Gasparoni, Gilles
Leidinger, Petra
Keller, Andreas
Meese, Eckart
Mayhaus, Manuel
Hampel, Harald
Riemenschneider, Matthias
External co-authors :
yes
Language :
English
Title :
The miRNome of Alzheimer's disease: consistent downregulation of the miR-132/212 cluster
Publication date :
2016
Journal title :
Neurobiology of Aging
ISSN :
1558-1497
Publisher :
Elsevier, New York, United States - New York
Volume :
50
Pages :
167
Peer reviewed :
Peer Reviewed verified by ORBi
Focus Area :
Systems Biomedicine
Available on ORBilu :
since 20 January 2017

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