Article (Scientific journals)
Transcriptomic analyses of primary astrocytes under TNFα treatment
Birck, Cindy; Koncina, Eric; Heurtaux, Tony et al.
2015In Genomics Data, 7, p. 7-11
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Keywords :
primary astrocytes; bioinformatics; TNF-alpha; inflammation; brain; NFkappaB; microarray; gene expression
Abstract :
[en] Astrocytes, the most abundant glial cell population in the central nervous system, have important functional roles in the brain as blood brain barrier maintenance, synaptic transmission or intercellular communications. Numerous studies suggested that astrocytes exhibit a functional and morphological high degree of plasticity. For example, following any brain injury, astrocytes become reactive and hypertrophic. This phenomenon, also called reactive gliosis, is characterized by a set of progressive gene expression and cellular changes. Interestingly, in this context, astrocytes can re-acquire neurogenic properties. It has been shown that astrocytes can undergo dedifferentiation upon injury and inflammation, and may re-acquire the potentiality of neural progenitors. To assess the effect of inflammation on astrocytes, primary mouse astrocytes were treated with tumor necrosis factor α (TNFα), one of the main pro-inflammatory cytokines. The strength of this study is that pure primary astrocytes were used. As microglia are highly reactive immune cells, we used a magnetic cell sorting separation (MACS) method to further obtain highly pure astrocyte cultures devoid of microglia. Here, we provide details of the microarray data, which have been deposited in the Gene Expression Omnibus (GEO) under the series accession number GSE73022. The analysis and interpretation of these data are included in Gabel et al. (2015). Analysis of gene expression indicated that the NFκB pathway-associated genes were induced after a TNFα treatment. We have shown that primary astrocytes devoid of microglia can respond to a TNFα treatment with the re-expression of genes implicated in the glial cell development.
Research center :
- Luxembourg Centre for Systems Biomedicine (LCSB): Biomedical Data Science (Glaab Group)
Luxembourg Centre for Systems Biomedicine (LCSB): Experimental Neurobiology (Balling Group)
Disciplines :
Neurology
Biotechnology
Physical, chemical, mathematical & earth Sciences: Multidisciplinary, general & others
Life sciences: Multidisciplinary, general & others
Author, co-author :
Birck, Cindy ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Koncina, Eric ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Heurtaux, Tony ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Glaab, Enrico  ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Michelucci, Alessandro ;  University of Luxembourg > Luxembourg Centre for Systems Biomedicine (LCSB)
Heuschling, Paul ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
Grandbarbe, Luc ;  University of Luxembourg > Faculty of Science, Technology and Communication (FSTC) > Life Science Research Unit
External co-authors :
no
Language :
English
Title :
Transcriptomic analyses of primary astrocytes under TNFα treatment
Publication date :
10 November 2015
Journal title :
Genomics Data
ISSN :
2213-5960
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
7
Pages :
7-11
Peer reviewed :
Peer Reviewed verified by ORBi
FnR Project :
FNR5782168 - Exploring Parkinson'S Disease Inhibitor Efficacy On A Non-dopaminergic Target, 2013 (01/12/2013-31/05/2016) - Enrico Glaab
Available on ORBilu :
since 16 November 2015

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