References of "1985"
     in
Bookmark and Share    
Peer Reviewed
See detailInfluence of acetylsalicylic and salicylic plasma levels on psychophysical measures of long-standing natural pain stimuli
Anton, Fernand UL; Handwerker, Hermann-Otto; Kreh, Albrecht et al

in Fields, Howard; Dubner, Ronald (Eds.) Advances in pain research and therapy 9 (1985)

Detailed reference viewed: 12 (0 UL)
Peer Reviewed
See detailInsertion of a bacterial gene into the mouse germ line using an infectious retrovirus vector.
Huszar, D.; Balling, Rudi UL; Kothary, R. et al

in Proceedings of the National Academy of Sciences of the United States of America (1985), 82(24), 8587-91

Using a Moloney leukemia virus vector containing the bacterial neo gene, we demonstrate that retrovirus vectors can be used to introduce genes into the mouse germ line. Infection of preimplantation ... [more ▼]

Using a Moloney leukemia virus vector containing the bacterial neo gene, we demonstrate that retrovirus vectors can be used to introduce genes into the mouse germ line. Infection of preimplantation embryos with the vector MLV-NEO.1 resulted in integration of neo sequences in approximately equal to 10% of the progeny mice. One of these animals, mouse F.2, contained approximately six MLV-NEO.1 proviruses at independent integration sites, each present at less than a single copy per cell. This mosaic mouse transmitted one of these proviruses to her offspring, producing a line of transgenic mice carrying a full-length, unrearranged MLV.NEO.1 provirus at a single chromosomal integration site. Mice homozygous at this MLV-NEO.1 locus have also been produced. No expression of the neo gene has been detected in the transgenic mice, either by screening of primary bone marrow or lung cells for resistance to G418 or by RNA transfer blot analysis of RNA from several tissues. In addition, the neo gene was found to be extensively methylated in the transgenic mice; however, treatment of primary cells with 5-azacytidine did not induce G418 resistance. The inactivity of the MLV-NEO.1 provirus in transgenic mice and potential means of eliciting neo expression under these conditions are discussed. [less ▲]

Detailed reference viewed: 49 (0 UL)
Peer Reviewed
See detailDirect effects of nicotine on rabbit preimplantation embryos.
Balling, Rudi UL; Beier, H. M.

in Toxicology (1985), 34(4), 309-13

The effects of various concentrations of nicotine on the in vitro development of 1-cell rabbit preimplantation embryos and on the DNA-synthesis of 4-day-old rabbit blastocysts are investigated. Exposure ... [more ▼]

The effects of various concentrations of nicotine on the in vitro development of 1-cell rabbit preimplantation embryos and on the DNA-synthesis of 4-day-old rabbit blastocysts are investigated. Exposure of rabbit preimplantation embryos to concentrations of nicotine higher than 1 X 10(-3) M results in a marked decrease in the in vitro development and in DNA-synthesis. Concentrations of nicotine below 1 X 10(-3) M have no effect on these early embryos. It can be concluded that the concentrations of nicotine which exert a direct embryotoxic effect are higher than the concentrations that may be expected in the blood circulation of humans considered to be "normal smokers". [less ▲]

Detailed reference viewed: 41 (0 UL)
Peer Reviewed
See detailOxidative and conjugative metabolism of diethylstilbestrol by rabbit preimplantation embryos.
Balling, Rudi UL; Haaf, H.; Maydl, R. et al

in Developmental Biology (1985), 109(2), 370-4

Five- and six-day-old rabbit preimplantation embryos were found to be capable of metabolizing [3H]diethylstilbestrol (DES) in vitro. Based on the analysis of the metabolites formed during a 24-hr ... [more ▼]

Five- and six-day-old rabbit preimplantation embryos were found to be capable of metabolizing [3H]diethylstilbestrol (DES) in vitro. Based on the analysis of the metabolites formed during a 24-hr incubation period we conclude that these early stage embryos do have active monooxygenase and conjugative activity. The monooxygenase seems to be specific to this early stage of embryonic development. [less ▲]

Detailed reference viewed: 45 (0 UL)
See detailSprachpolitik in Katalonien
Ehrhart, Sabine UL

Bachelor/master dissertation (1985)

Detailed reference viewed: 54 (1 UL)
Full Text
Peer Reviewed
See detailPhosphorylation of the Ca2+-pumping ATPase of heart sarcolemma and erythrocyte plasma membrane by the cAMP-dependent protein kinase.
Neyses, Ludwig UL; Reinlib, L.; Carafoli, E.

in The Journal of biological chemistry (1985), 260(18), 10283-7

The Ca2+ ATPase of heart sarcolemma was stimulated by the exposure of sarcolemma vesicles to ATP and the catalytic subunit of the cAMP-dependent protein kinase. The effect of the phosphorylation system ... [more ▼]

The Ca2+ ATPase of heart sarcolemma was stimulated by the exposure of sarcolemma vesicles to ATP and the catalytic subunit of the cAMP-dependent protein kinase. The effect of the phosphorylation system was primarily on the Km(Ca2+) of the pumping ATPase. The ATPase purified from heart sarcolemma or erythrocytes became phosphorylated under the conditions mentioned above. Hydroxylamine treatment of the labeled ATPase has shown that the phosphorylation was additive to be acylphosphate formed on the ATPase during the reaction cycle. The stoichiometry of the kinase-promoted phosphorylation (i.e. the fraction of the ATPase molecules that became labeled) approached 30% with both the heart and the erythrocyte enzyme. [less ▲]

Detailed reference viewed: 86 (0 UL)
Full Text
Peer Reviewed
See detailStereospecific modulation of the calcium channel in human erythrocytes by cholesterol and its oxidized derivatives.
Neyses, Ludwig UL; Locher, R.; Stimpel, M. et al

in The Biochemical journal (1985), 227(1), 105-12

To study the effect of cholesterol and its pathophysiologically important oxidized derivatives (OSC) on the calcium entry channel, the human red blood cell was used as a model system. The calcium ejecting ... [more ▼]

To study the effect of cholesterol and its pathophysiologically important oxidized derivatives (OSC) on the calcium entry channel, the human red blood cell was used as a model system. The calcium ejecting adenosinetriphosphatase (ATPase) was inhibited by vanadate. The cells were loaded with OSC at concentrations between 1.25 X 10(-5) and 25 X 10(-5) mol/l. 22-Hydroxycholesterol, cholestan-3 beta,5 alpha,6 beta-triol, 5 alpha-cholestan-3 beta-ol,3 beta,5 alpha-dihydroxycholestan-6-one and 3 beta-hydroxy-5 alpha-cholestan-7-one stimulated 45Ca2+ influx by up to almost 90%, whereas 25-hydroxycholesterol, 7 beta-hydroxycholesterol, 20 alpha-hydroxycholesterol and 7-oxocholesterol inhibited influx by up to 75%. Both stimulation and inhibition were dependent on the amount of OSC incorporated into the membrane. More than 90% of the total modification of calcium influx by OSC was accounted for by an influence on the nitrendipine-inhibitable part of influx. Enrichment of cholesterol in the membrane greatly stimulated, and cholesterol depletion inhibited, Ca2+ influx. These results demonstrate that cholesterol and its oxidized derivatives are able to modulate the calcium channel in human red blood cells in a highly stereospecific manner. [less ▲]

Detailed reference viewed: 36 (2 UL)
See detailYouth Conflicts and Public Policy Challenges in Western Europe
Willems, Helmut UL; Eckert, Roland

in XXth International CFR Seminar on Social Change and Family Policies, Melbourne, Australia, 19-24 Aug 1984 (1985)

Detailed reference viewed: 12 (0 UL)
Peer Reviewed
See detailA simple and effective method to teach patients about high blood pressure and obesity.
Neyses, Ludwig UL; Greminger, P.; Bartsch, A. et al

in Journal of hypertension. Supplement : official journal of the International Society of Hypertension (1985), 3(1), 27-30

It is an open question whether information about hypertension and obesity increases compliance with therapy. Nevertheless, patients increasingly demand precise but simple and comprehensive information. A ... [more ▼]

It is an open question whether information about hypertension and obesity increases compliance with therapy. Nevertheless, patients increasingly demand precise but simple and comprehensive information. A simple slide programme is described which can be demonstrated in any waiting room. The learning effect was assessed in 1083 subjects, of whom 485 had seen the programme completely; 256 subjects served as controls. The percentage of subjects with good or excellent knowledge about hypertension and obesity rose from 22.8% in the controls to 64.2% in the experimental group. Age was the only factor influencing learning, but this was of no great importance in subjects under 70. In particular, social status did not have any significant effect on learning. This programme may be an ideal tool to inform patients about hypertension and obesity and to study the influence of information on compliance with therapy. [less ▲]

Detailed reference viewed: 19 (5 UL)
Peer Reviewed
See detailDoes continuous subcutaneous insulin infusion (CSII) prolong the remission phase of insulin-dependent diabetic children? Preliminary findings of a randomized prospective study
De Beaufort, Carine UL; Bruining, G. J.; Aarsen, R. S. R. et al

in Netherlands Journal of Medicine (1985), 28(SUPPL. 1), 53-54

[No abstract available]

Detailed reference viewed: 4 (0 UL)
Peer Reviewed
See detail[Mechanism of action of calcium antagonists].
Stimpel, M.; Neyses, Ludwig UL

in Schweizerische Rundschau fur Medizin Praxis = Revue suisse de medecine Praxis (1985), 74(19), 483-90

Detailed reference viewed: 15 (0 UL)
Peer Reviewed
See detailTrilostane - a new and effective drug for primary aldosteronism
Neyses, Ludwig UL; Groth, H; Vetter, H et al

in The adrenal gland and hypertension (1985)

Detailed reference viewed: 15 (1 UL)
Peer Reviewed
See detailThe mechanism of action of calcium antagonists [DER WIRKUNGSMECHANISMUS DER KALZIUMANTAGONISTEN]
Stimpel, M.; Neyses, Ludwig UL

in Schweizerische Rundschau fur Medizin/Praxis (1985), 74(19), 483-490

Detailed reference viewed: 18 (0 UL)
Peer Reviewed
See detail[Divisible tablets--source of error in therapeutic drug dosages].
Stimpel, M.; Vetter, H.; Kuffer, B. et al

in Schweizerische Rundschau fur Medizin Praxis = Revue suisse de medecine Praxis (1985), 74(5), 84-6

Detailed reference viewed: 22 (0 UL)
Peer Reviewed
See detailCompliance with salt restriction as a limiting factor in the primary prevention of hypertension.
Neyses, Ludwig UL; Dorst, K.; Michaelis, J. et al

in Journal of hypertension. Supplement : official journal of the International Society of Hypertension (1985), 3(1), 87-90

It is an important but still unresolved question whether reduction of salt intake in the offspring of hypertensives (a high risk group) prevents the development of the disease. Therefore, 178 offspring ... [more ▼]

It is an important but still unresolved question whether reduction of salt intake in the offspring of hypertensives (a high risk group) prevents the development of the disease. Therefore, 178 offspring (14-26 years old) of hypertensives were enrolled in a 2-year pilot trial aimed mainly at a reduction in salt consumption. For the intervention group (n = 99) a behavioural approach was chosen with extensive counselling by experienced dietitians. The controls (n = 79) received no continuous dietary advice. Both groups showed a small decline in sodium intake over time, but the differences between the two groups were not significant. Division into subgroups with and without sodium reduction revealed no differences in blood pressure. We conclude that the inherent resistance to any change of lifestyle among healthy subjects may require new and more comprehensive motivational approaches. [less ▲]

Detailed reference viewed: 21 (0 UL)
Full Text
Peer Reviewed
See detailNonlinear optical properties of Rb2ZnCl4 in the incommensurate and ferroelectric phases
Sanctuary, Roland UL; Jundt, D.; Baumert, J. et al

in Physical Review. B, Condensed Matter and Materials Physics (1985), 32(3), 1649-1660

Nonlinear optical properties of Rb2ZnCl4 are measured for the incommensurate and ferroelectric phases by means of second-harmonic generation experiments. In the temperature range of the ferroelectric lock ... [more ▼]

Nonlinear optical properties of Rb2ZnCl4 are measured for the incommensurate and ferroelectric phases by means of second-harmonic generation experiments. In the temperature range of the ferroelectric lock-in phase, the nonlinear optical coefficients d33, d32, and d24 and the Miller coefficients are determined using the wedge technique and are shown to be proportional to the spontaneous polarization. In the incommensurate phase of Rb2ZnCl4, the order of magnitude of the second-harmonic intensity corresponding to nonlinear optical coefficients allowed by the local point-group symmetry is estimated to be at least 107 times smaller than the second-harmonic intensity generated in quartz and thus difficult to detect. Furthermore, it is shown that quasi-phase-matching of the fundamental and second-harmonic waves in the incommensurate phase is limited to a very small temperature range of TC [less ▲]

Detailed reference viewed: 29 (0 UL)
Peer Reviewed
See detailHigh density lipoproteins--modulators of the calcium channel?
Stimpel, M.; Neyses, Ludwig UL; Locher, R. et al

in Journal of hypertension. Supplement : official journal of the International Society of Hypertension (1985), 3(3), 49-51

It has recently been shown that human red blood cells possess a voltage-independent calcium channel which can be influenced by in vitro modification of the membraneous cholesterol content. To determine ... [more ▼]

It has recently been shown that human red blood cells possess a voltage-independent calcium channel which can be influenced by in vitro modification of the membraneous cholesterol content. To determine whether there is also a link between plasma lipids and the calcium influx through this channel under in vivo conditions, the calcium influx was measured in red blood cells from 51 male donors (aged 41 +/- 5 years). The influx through the channel was defined as the nitrendipine (15 mumol/l)-inhibitable part of 45Ca2+ influx. The Ca(2+)-ejecting ATPase was inhibited by vanadate. The results demonstrate a strong inverse relationship (r = -0.81; P < 0.001) between the plasma concentration of high density lipoproteins (HDL) and 45Ca2+ influx. No significant correlation was found between 45Ca2+ influx and triglycerides, low density lipoproteins (LDL), very low density lipoproteins (VLDL), total plasma cholesterol or extracellular electrolytes (K+, Na+, Ca2+, Mg2+). The results indicate that HDL are involved in the modulation of the calcium channel and provide a link between the cellular cholesterol turnover and the calcium influx in the pathogenesis of atherosclerosis and hypertension. [less ▲]

Detailed reference viewed: 15 (0 UL)