References of "Ardini, Matteo"
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See detailOn-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes
Huang, Jian-An; Caprettini, Valeria; Zhao, Yingqi et al

in Nano Letters (2019), 19(2), 722-731

Delivery of molecules into intracellular compartments is one of the fundamental requirements in molecular biology. However, the possibility of delivering a precise number of nano-objects with single ... [more ▼]

Delivery of molecules into intracellular compartments is one of the fundamental requirements in molecular biology. However, the possibility of delivering a precise number of nano-objects with single-particle resolution is still an open challenge. Here we present an electrophoretic platform based on 3D hollow nanoelectrodes to enable delivery of single nanoparticles into single selected cells and monitoring of the single-particle delivery by surface-enhanced Raman scattering (SERS). The gold-coated hollow nanoelectrode capable of confinement and enhancement of electromagnetic fields upon laser illumination can distinguish the SERS signals of a single nanoparticle flowing through the nanoelectrode. Tight wrapping of cell membranes around the nanoelectrodes allows effective membrane electroporation such that single gold nanorods are delivered on demand into a living cell by electrophoresis. The capability of the 3D hollow nanoelectrodes to porate cells and reveal single emitters from the background in continuous flow is promising for the analysis of both intracellular delivery and sampling. [less ▲]

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See detailLive Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles Clusters
Ardini, Matteo; Huang, Jian-An; Sanchez, Carlos S. et al

in Scientific Reports (2018), 8

Live intracellular imaging is a valuable tool in modern diagnostics and pharmacology. Surface Enhanced Raman Spectroscopy (SERS) stands out as a non-destructive and multiplexed technique, but ... [more ▼]

Live intracellular imaging is a valuable tool in modern diagnostics and pharmacology. Surface Enhanced Raman Spectroscopy (SERS) stands out as a non-destructive and multiplexed technique, but intracellular SERS imaging still suffers from interfering background from endogenous components. Here we show the assembly of small colloidal SERS probes with Raman signal in the cell-silent window of 1800–2900 cm−1 for biorthogonal intracellular SERS imaging of dopamine that was undistinguishable from the endogenous cell background. By linking colloidal silver nanoparticles with alkyne-dopamine adducts, clusters are formed by 2–6 nanoparticles spaced by tight interparticle gaps that exhibited high electric field enhancement and strong SERS signals of alkyne and dopamines. Due to the cell-silent signals of the alkyne, intracellular in-vitro Raman imaging shows that the dopamines on the internalized clusters remain distinguishable across the cytoplasm with good spatial resolution. Our method can be a general-purpose method for real-time imaging of biomolecules, such as proteins, peptides, DNA and drugs. [less ▲]

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See detailHybrid plasmonic nanostructures based on controlled integration of MoS2 flakes on metallic nanoholes
Garoli, Denis; Mosconi, Dario; Miele, Ermanno et al

in Nanoscale (2018), 10(36), 17105-17111

Here, we propose an easy and robust strategy for the versatile preparation of hybrid plasmonic nanopores by means of controlled deposition of single flakes of MoS2 directly on top of metallic holes. The ... [more ▼]

Here, we propose an easy and robust strategy for the versatile preparation of hybrid plasmonic nanopores by means of controlled deposition of single flakes of MoS2 directly on top of metallic holes. The device is realized on silicon nitride membranes and can be further refined by TEM or FIB milling to achieve the passing of molecules or nanometric particles through a pore. Importantly, we show that the plasmonic enhancement provided by the nanohole is strongly accumulated in the 2D nanopore, thus representing an ideal system for single-molecule sensing and sequencing in a flow-through configuration. Here, we also demonstrate that the prepared 2D material can be decorated with metallic nanoparticles that can couple their resonance with the nanopore resonance to further enhance the electromagnetic field confinement at the nanoscale level. This method can be applied to any gold nanopore with a high level of reproducibility and parallelization; hence, it can pave the way to the next generation of solid-state nanopores with plasmonic functionalities. Moreover, the controlled/ordered integration of 2D materials on plasmonic nanostructures opens a pathway towards new investigation of the following: enhanced light emission; strong coupling from plasmonic hybrid structures; hot electron generation; and sensors in general based on 2D materials. [less ▲]

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See detailNanoporous gold decorated with silver nanoparticles as large area efficient SERS substrate
Ardini, Matteo; Huang, Jian-An; Sanchez-Sanchez, Carlos et al

in Proceedings of SPIE : The International Society for Optical Engineering (2017), 10346

Nanoporous gold is a very promising material platform for several plasmonic applications. Nanoporous gold can be formed by dealloying Au–Ag alloys, previously grown by means of Ag-Au co-sputtering. The ... [more ▼]

Nanoporous gold is a very promising material platform for several plasmonic applications. Nanoporous gold can be formed by dealloying Au–Ag alloys, previously grown by means of Ag-Au co-sputtering. The optical response is completely determined by the nanostructured film features, that only depend on the initial alloy composition. It has been extensively used as SERS substrate both as thin film and nanofabricated fancy designs. Here we explore the potential application of nanoporous gold as SERS substrate as it is coupled and decorated with Ag nanoparticles. Significant enhancement has been observed in comparison with bare nanoporous film. [less ▲]

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